Device

Part:BBa_M45138:Design

Designed by: Ozkan Fidan   Group: UtahState_BE5930_-_S14   (2014-04-15)

Type II Secretion of Chromate and Uranium Reductase with TorA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 918
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 685
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ChrR30 and ChrR6 are improved by directed evolution.


Source

References

Barak Y et al. “Analysis of Novel Soluble Chromate and Uranyl Reductases and Generation of an Improved Enzyme by Directed Evolution” Applied and Environmental Microbiology, 2006;72:7074-7082 Barak Y et al. “ Enzyme Improvement in the Absence of Structural Knowledge: a Novel Statistical Approach” International Society for Microbial Ecology, 2008;2:171-179